• Event
  • Scientific training

Flow Cytometry Training

Improve theoretical knowledge in flow cytometry to assist with planning and running your own experiment

Start date:

26 February 2019

End date:

26 February 2019

Time:

09h00 - 17h00

Venue:

Earlham Institute

Registration deadline:

15 January 2019

Cost:

£120

About the event.

Aim:

Improve theoretical knowledge in flow cytometry to assist with planning and running your own experiment

Learning Outcomes:

  1. Gain an understanding of how fluorochromes behave and considerations when planning a multicolour panel experiment
  2. Understand the importance of controls and how compensation works
  3. Understand how a cytometer works
  4. Gain confidence to diagnose problems and how to remedy them
  5. Develop knowledge of what CS&T and accudrop beads do
  6. Have broadened understanding of important considerations for single cell sorting
  7. Have the opportunity to discuss your experimental planning with experienced cytometrists

Depending on applicants’ interests, there may be time to cover additional topics of common interest, for example cell sorting experiments, so please highlight these in your application.

Prerequisites:

No previous experience required. Preference will be given to those in the early planning stages of their projects.

Target Audience:

Ideal for researchers starting out in flow cytometry and in the planning stages of an experiment. An opportunity to expand theoretical knowledge for those more experienced researchers.

Programme.

26 February 2019

Time

Topic

08:30 - 09:00

Coffee

09:00 - 09:30

Welcome and EI projects

09:30 - 10:30

Fluorescence and Fluorochromes

Understand the principles of fluorescence, excitation and emission spectra, different classes of dyes used in fluorescence technologies.

10:30 - 11:00

Coffee/Tea

11:00 - 12:00

How a Flow Cytometer works

The fluidics, optics and electronics systems found on all flow cytometers. How to use this knowledge to help troubleshoot.

12:00 - 12:30

Introduction to Compensation

How flourescence spillover can be corrected; the right controls to bring to the cytometer to ensure successful compensation

12:30 - 13:30

Lunch

13:30 - 14:30

Cell sorting

The principles of droplet sorting using the FACS Melody as an example including Quality Control and correctly setting drop delay. Applications of cell sorting.

14:30 - 15:00

Experimental Design and Sample Preparation

How to use knowledge of fluorescence & the cytometer to design successful multicolour experiments. Which factors need to be considered in running an experiment (sample prep, staining, controls).

15:00 - 15:30

Coffee

15:30 - 16:30

Data Analysis and presentation

How to select the cells that you are interested in. The principles of gating and deriving the most meaningful statistic. How to present flow data to an audience.

16:30 - 17:00

Q&A on specific questions

17:00 - 17:15

Feedback and Close

Fees and accommodation.

Registration includes lunch and refreshments, plus access to training materials.