Event Scientific training

Introduction to Flow Cytometry

Improve theoretical knowledge in flow cytometry to assist with planning and running your own experiment

Start date: 19 June 2024
End date: 19 June 2024
Time: 09.00 - 17.15
Venue: Earlham Institute (Norwich UK)
Organiser: Iain Macaulay



Registration deadline: 18 May 2024
Cost: £175

About the event

The workshop will improve theoretical knowledge in flow cytometry including:

  • Understanding of fluorescence and fluorochromes: principles of fluorescence, excitation and emission spectra, different classes of dyes used in fluorescence technologies. 
  • Understand how a flow cytometer works: The fluidics, optics and electronics systems found on all flow cytometers; How to use this knowledge to help troubleshoot;
  • Considerations of conventional and full spectrum cytometry
  • Gain knowledge in compensation and data spread: How fluorescence spillover can be corrected; the right controls to bring to the cytometer to ensure successful compensation or unmixing
  • Best practice in experimental design and sample preparation: How to use knowledge of fluorescence and the cytometer to design successful multicolour experiments;
  • Which factors need to be considered in running an experiment (sample preparation, staining, controls, quality control)
  • The principles of droplet sorting using the FACS Melody as an example including Quality Control and correctly setting drop delay
  • Applications of cell sorting
  • How to select the cells that you are interested in
  • Learn the principles of gating and deriving the most meaningful statistic
  • How to present flow data to an audience
  • Learn of new technologies: Including BD S8



No previous experience required. Preference will be given to those in the early planning stages of their projects.

Target Audience:

Ideal for researchers starting out in flow cytometry and in the planning stages of an experiment. An opportunity to expand theoretical knowledge for those more experienced researchers.

Register today.

Registration deadline: 18 May 2024

Participation: First come, first served