Earlham Biofoundry

Working with us.

Led by: Biofoundry Manager

Jose A. Carrasco Lopez

The Earlham Biofoundry is a resource for the UK biology and biotechnology communities, providing a platform to undertake large-scale projects. We house suites of laboratory automation that, together with our expertise in large-scale experimentation and synthetic biology, can be applied to numerous molecular and microbiological workflows.

Synthetic Biology applies engineering principles such as standardisation and modularisation to biological sciences through the application of iterative design-build-test-learn cycles. The standardisation of biological components and reactions allows experimental workflows to be automated. This offers the potential to revolutionise the speed and scale of research, increasing accuracy and allowing miniaturisation to reduce costs. The adoption of these principles and approaches enables scientists to pursue complex experimental designs.

Established automated workflows are available to researchers across academia and industry and can be requested as a service. Alternatively, we can work collaboratively, contributing our scientific expertise and technical knowledge to develop new methods, automate and scale-up workflows and apply synthetic biology approaches to scientific problems.We also provide training in laboratory automation through practical training sessions, laboratory placements, training courses and by hosting collaborators within the biofoundry.

To discuss a project or collaboration, get in touch with us today.

We welcome all enquiries from academia and industry. We would be happy to provide our expertise and facilities for the development of cust customized workflows or automation training.

To request more information and scientific or technical support, or quotes for services or grant applications, please contact our Biofoundry Manager:

jose.carrasco-lopez@earlham.ac.uk

Collaborative projects

We specialise in the rapid design, construction, and testing of genetically reprogrammed organisms. However, we have worked on a range of different projects contributing our expertise in laboratory automation and synthetic biology approaches to experimentation to optimise experiment design, increase throughput, reduce reaction volumes and improve reproducibility. We have worked with a range of different partners in academia and industry.

Open quote marks

Working with the Earlham Institute allows us to really quickly scale up our Research and Development, this way we have the right people to not just help us do it but teach us how to do it better.

Closing quote marks

Orr Yarkoni, CEO, Colorifix

Service workflows

We offer access to a number of highly-optimised automated workflows on a fee-for-service basis. These are performed on our state-of-the-art automation platforms including:

  • Nanoscale DNA assembly of plasmid constructs from standard parts including the construction of combinatorial libraries
  • Validation of DNA assemblies by next generation sequencing
  • Bacterial transformation
  • Microfermentation, including condition optimisation (aerobic and anaerobic)
  • Colony picking and arraying
  • Plasmid purification
  • Generation of knock-out libraries
  • DNA quantification and normalisation
  • Cell-free protein expression
  • Automated DNA electrophoresis (eGels)
  • ELISA
  • Creation and curation of stocks
  • Automated sample storage and retrieval
  • Nanoscale PCR and qPCR
  • PCR clean-up

We can also collaborate to automate additional protocols and workflows using one or more of our automation platforms:

DNA Assembly

Parallel DNA assembly methods allow multiple fragments of DNA to be compiled in a desired order in a single reaction.

Several methods enable the efficient one‐step assembly of multiple DNA parts into a suitable plasmid acceptor at high efficiency. These allow multiple plasmids housing standardized DNA parts of interest are combined in an enzyme cocktail.

The EI Biofoundry can work with most assembly methods and plasmid toolkits. Please note, to use plasmids provided by a third party you will require an MTA with the originating party. We have completed projects in the EI Foundry using the following toolkits.

Click Here to acess the repository of standard DNA Parts

Publication: in review

Access to the standard DNA assebly yet to be confirmed.

Plasmids available from Addgene: In process