Advanced sequencing

From microbial genomes, all the way up to massively polyploid plants, we are experienced in a number of whole genome sequencing approaches across all our sequencing platforms. Whether you’re resequencing a model organism, performing population-scale diversity studies, or need a single high-quality genome assembly of your favourite organism we have an approach that will suit your work from Pacific Biosciences SMRT-Sequencing to ultra-high throughput sequencing on the HiSeq.

If whole genome sequencing is beyond the budget, or you simply don’t need all that information, then targeted sequencing approaches allow you to focus on the regions of the genome you are most interested in. We are experienced in supporting in-solution hybridisation capture approaches from small panels on the MiSeq to exome-scale studies on HiSeq platforms. Custom amplicon designs can also be run. We can advise on targeted panel design and analysis, as well as provide the sequencing.

We can provide a number of approaches to transcriptome sequencing and analysis from short-read based transcript reconstruction approaches on the HiSeq to Iso-Seq full-length transcript sequencing on our PacBio platforms. Whether you’re looking to generate a reference transcriptome by assembly, looking at differential expression or looking for gene fusions talk to us about our approaches. We also offer smallRNA and microRNA sequencing for discovery or differential expression studies.

Being able to look at the transcriptional dynamics, or genetic variation between single cells is a powerful new tool in genomics. We have multiple single-cell platforms to provide DNA-Seq or RNA-Seq from single cell suspensions. We have close links to our Faculty single-cell expert and can provide advice on the successful setup and analysis of single-cell experiments.

Community profiling of microbiomes can be done in a number of ways. We offer 16S and 18S metagenomics approaches (other amplicon approaches are available) for rapid interrogation of communities from anything from human to environmental samples. If you’re looking for more information on the biochemical pathways present in a community we can also offer whole genome shotgun metagenomics as well as metatranscriptomics approaches.