
Biography
Contact details:
I am an undergraduate student studying Biological Sciences at Durham University, currently here at EI as a year in industry student. During this year, I hope to discover more about genomics, an interest that was sparked during my first two years of my degree. I hope to use the year to expand my knowledge in the field and apply the theories and techniques I have learned previously to my own research project.
My year in industry project is a collaboration between multiple faculty groups: the Macaulay, Haerty and Swarbreck groups and Genomic Pipelines. Over the past decade, significant advances have been made in single-cell RNA sequencing. Smart-seq3 is a new single-cell RNA sequencing protocol published by Hagemann-Jenson et al., 2020; I will be working to establish a Smart-seq3 pipeline within EI. This will involve performing a comparison between Smart-seq3 and Smart-seq2 (the current protocol used), for their use in single-cell analysis of human cancer cell lines to assess gene expression heterogeneity.
I will then work towards building a 384-well plate Smart-seq3 pipeline, which could potentially enable parallel sequencing of up to 1,536 cells in a single experiment. During my project I will gain experience in cell sorting, method development, laboratory automation, next generation sequencing and the associated bioinformatic skills to enable me to analyse my own sequencing data.
Related reading.

Bananas are on the brink but close cousins could save their skins

Cultural differences: how analysing mixed communities of microorganisms could help us understand AMR

Memories of sequencing the human genome to mark seven decades of DNA

Hidden jewels in our blood could hold secrets of healthy ageing

Differences make a difference: from one cell to a world of individuality

Artificial realities: copying wild microbial communities in the lab

New insights into resistance to antimicrobials could stop bacteria in their tracks

Engineered plants produce sex perfume to trick pests and replace pesticides

Human body a breeding ground for antimicrobial resistance genes

Key tilapia genome offers boost to global food security

Exotic wheat DNA could help breed ‘climate-proof’ crops

Sequencing project to unleash the huge potential of euglenoids

Circadian clock insights could be key to increased wheat yields

European consortium launched to reverse biodiversity loss through genomics research

Tracking bacterial evolution in real time spots emergence of antimicrobial resistance
