

Eleanor Calcutt
Biography
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Biography
I am an undergraduate student studying Biological Sciences at Durham University, currently here at EI as a year in industry student. During this year, I hope to discover more about genomics, an interest that was sparked during my first two years of my degree. I hope to use the year to expand my knowledge in the field and apply the theories and techniques I have learned previously to my own research project.
My year in industry project is a collaboration between multiple faculty groups: the Macaulay, Haerty and Swarbreck groups and Genomic Pipelines. Over the past decade, significant advances have been made in single-cell RNA sequencing. Smart-seq3 is a new single-cell RNA sequencing protocol published by Hagemann-Jenson et al., 2020; I will be working to establish a Smart-seq3 pipeline within EI. This will involve performing a comparison between Smart-seq3 and Smart-seq2 (the current protocol used), for their use in single-cell analysis of human cancer cell lines to assess gene expression heterogeneity.
I will then work towards building a 384-well plate Smart-seq3 pipeline, which could potentially enable parallel sequencing of up to 1,536 cells in a single experiment. During my project I will gain experience in cell sorting, method development, laboratory automation, next generation sequencing and the associated bioinformatic skills to enable me to analyse my own sequencing data.
Related reading.

On the origin of errors: the causes and consequences of mistakes during DNA replication

Why is genome annotation important?

Why cloud computing is important for data-driven bioscience research

How bioinformatics can crack the complex case of protist biodiversity

The dramatic effects genomics will have on our future world

The inextricable link between climate change and biodiversity.

Hidden Biodiversity: How genomics can save nature’s secrets before they’re lost forever

Can we reach net zero & climate resilience? Here are 6 ways Earlham scientists are getting us there

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Precision medicine tool finds hidden genetic connections that could personalise IBD treatment

Deciphering gut microbiome ‘chatter’ to combat IBD

New legislation granted to progress plant gene editing in UK

'Molecular Facebook’ signals researchers to right path for understanding cell function in disease

UK project underpins global effort to map genomes of all life on earth
