Detection of DNA base modification using nanopore sequencing

New for 2024, in this comprehensive two-day course, participants will learn how to use Oxford Nanopore Technologies (ONT) sequencing to detect DNA base modifications.

The course covers experimental design, including important considerations for generating ultra long Nanopore sequencing reads. Through a blend of lectures and hands-on sessions, attendees will gain the skills necessary to analyse base modification profiles in various contexts.

We will also explore relevant research case studies and practice visualising and manipulating base modification data using genome browsers and other tools.

What will I learn?

• Principles of base modification detection using Nanopore sequencing
• Experimental considerations for generating ultra long Nanopore sequencing reads
• Sequence alignment (minimap2) and data QC (pycoQC)
• Hands-on experience with base modification detection (Nanopolish, DNAscent, Remora)
• Visualisation and manipulation base modification data (IGV, modBAM)
• Comparison of single molecule base modification detection approaches 

Who is this event for?

Early career researchers who are in the planning stages of a research project looking at base modification detection. 

Course prerequisites

You should have familiarity with the command line and either be familiar with actions as in the Software Carpentry lesson on The Unix Shell; or work through these before the training course starts.

Above Image: Oxford Nanopore Technologies, February 2023.