Introduction to Single-Cell RNAseq
From single-cell isolation, to library construction, sequencing and bioinformatics analysis
The course will provide an introduction to Single Cell Genomics. It covers several aspects of single-cell workflows from experimental design to laboratory hands-on including cell sorting and processing for production of quality samples for sequencing, generation of sequencing data and will introduce tools and metrics for assessing the data quality.
Laboratory practicals will involve cell sorting using FACS AriaFusion, including considerations for different cell types, preparation of samples for Smart-seq, library construction, QC and pooling, loading of the 10X instrument.
Delegates will have the opportunity to review sequencing reports to determine data quality, interpret library and pre-processing QC, understand the principles of producing matrices, and identify any filtering required. All theory lectures and hands-on sessions will include best practices and tips as learned first-hand by EI's own faculty.
The course will consist of a mixture of conceptual lectures, methodological lectures and hands-on sessions in the laboratory and review the steps of computational analyses, as well as ample time for group discussions.
Wet-lab researchers. Early career researchers (PhD and early career PostDocs), research assistants and technicians. Those in the experimental planning stages of a project involving single-cell genomics.
Please see our course prerequisites for further information.
Registration includes lunch and refreshments throughout the course, access to training materials and attendance at the course dinner.
Please note that the programme is in draft and therefore subject to changes before September.
Welcome and Course Overview
Flash presentations from participants
Experimental design including choosing methods
(Smartseq, G&T Seq, 10X, controls)
Hands-on: Cell Sorting and QC
Demonstration and discussion: Counting Nuclei
Hands-on: Smart-seq up to RT
Demonstration: 10X Chromium X
Close of Day 1
Hands-on: post-PCR clean up
cDNA sample QC (Qubit)
(coffee available from 10:30)
QC summary, Bioanalyzer
Hands-on: Library Prep (Nextera)
Hands-on: Library pooling and QC (QuBit)
Lecture: Sequencing platforms for SC RNAseq: Gold standard vs novel
Close of Day 2
Introduction to informatics
Library and pre-processing QC
Group work: troubleshooting data
Group discussion: What’s next for Single-Cell?
Results discussion and Q&A
Feedback and close of Day 3
Terms and Conditions:
Please carefully review our standard online event booking terms and conditions prior to registering for this event. Completing an online registration and associated payment process will mean that you are bound by these terms and conditions. Any supplemental terms or changes to these conditions on a per event basis will be included on this page.
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Registration deadline: 18 August 2023