Nanopore metagenomics 2023: from sample to analysis
In this in-person workshop, learn how to extract DNA from metagenomic samples,. prepare libraries, carry out nanopore sequencing and analyse data.
The goal of this course is to provide an overview of lab and in-field, real-time nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform.
This course will cover theory of experimental methods and sample collection, plus theory and hands-on experience of DNA extraction and library preparation.
The second part of the course will cover data analysis and visualisation providing hands-on opportunities including the use of QC tools, EI’s MARTi software and tools for Metagenome Assembled Genomes (MAGs), all delivered via virtual machines. You will also have the opportunity to discuss and apply lessons to your own projects with EI’s experts during an optional Q&A session.
What will I learn?
Who is this training for?
You should be in the early planning stages of a project, wanting to understand the different approaches to sample preparation and explore options for comprehensive and rapid data analysis.
Some basic level of experience with the Linux command line is useful, but not essential.
Necessary commands will be provided throughout the hands-on sessions.
Welcome and Flash Presentations
Course Overview and Introductory Lecture
Lecture: DNA extraction
Hands on: DNA extraction and loading tapestation
Lecture: Library construction
Hands on: Making a RAPID library
Hands on: Loading the sequencer
Lecture: Overview of Bioinformatics
Lecture: Quality control and assembly
Lecture: Metagenomic Classification with epi2me, MEGAN and MARTi
Hands on: QC and MARTi
Hands on: Assembly
Q&A with participants' projects
Hands on: Review assembly
Wrap up and feedback
This event is offered in-person on a first-come, first-served basis. We will review this in February 2023, and may open for virtual registrations.
Please carefully review our standard online event booking terms and conditions prior to registering for this event. Completing an online registration and associated payment process will mean that you are bound by these terms and conditions. Any supplemental terms or changes to these conditions on a per event basis will be included on this page. If you have any queries regarding our events or in relation to your booking, please contact us at email@example.com
You will find information about travelling to Earlham Institute and accommodation options here.
Registration deadline: 12 June 2023
Participation: First come, first served