Nanopore metagenomics: from sample to analysis 2022

What is the workshop about?

The goal of this course is to provide an overview of in-field, real-time nanopore sequencing using the Oxford Nanopore Technologies (ONT) platform. This course will cover experimental considerations, sample collection and preparation theory, plus data analysis and visualisation. Hands-on opportunities for data analysis of metagenomic data will cover the use of EI’s MARTi software and will be provided via virtual machines. You will also have the opportunity to discuss and apply lessons to your own projects with EI’s experts and a representative from Oxford Nanopore Technologies during an optional Q&A session.

What will I learn?

• Experimental considerations for nanopore sequencing
• Advantages and disadvantages of different extraction protocols
• Familiarity with different DNA QC and library construction methods
• Familiarity with nanopore sequencing outputs
• Common analysis tools for alignment, assembly and classification of nanopore data
• Capabilities of nanopore’s epi2me cloud-based data analysis platform
• Effective use of MARTi software for real-time data analysis for species identification and quantification

Who is this event for?

You should be in the early planning stages of a project, wanting to understand the different approaches to sample preparation and explore options for comprehensive and rapid data analysis. 

Course prerequisites

Some basic level of experience with the Linux command line is useful, but not essential. Necessary commands will be provided throughout the hands-on sessions. 

You MUST attend a pre-course drop-in session to enable us to provide access to your virtual machine for the course, typically two weeks before the course start-date.

This training forms part of our BBSRC National Capability in Advanced Training