• Research

Conrad Nieduszynski

Group Leader

Conrad Nieduszynski

Conrad is a genome biologist interested in how DNA replication impacts on genome evolution and stability. His group’s research is focused on combining cutting-edge genomic technologies with systems-level mathematical models and synthetic genomes to identify the mechanisms regulating DNA replication.

Conrad obtained his PhD in biochemistry (University of Cambridge) for work that included whole genome analysis of the genes controlling animal cell division. In post-doctoral research at the Universities of Dundee and Aberdeen, he used comparative genomics to identify DNA replication initiation sites. Conrad was awarded a Leverhulme Trust fellowship in 2006 and a BBSRC David Phillips Fellowship in 2008, which allowed him to establish his own research group, first at the University of Nottingham and subsequently at the University of Oxford. Conrad joined EI in 2020.

Conrad’s research group have pioneered genomic, computational and systems biology approaches to quantitatively measure how genomes replicate – both in populations of cells and on single molecules. This research has driven fundamental discoveries about how genomes replicate in bacteria, archaea and eukaryotes. Highlights include: the first single molecule sequencing method for the study of genome replication; the discovery that at least one organism does not require replication origins for rapid growth; mechanisms that ensure stable chromosome inheritance; and how synthetic chromosomes can be designed to ensure stable replication.

Publications.

Google Scholar

The Beacon Calculus: A formal method for the flexible and concise modelling of biological systems

Boemo, M., Cardelli, L., Nieduszynski, C. (2020). PLOS Computational Biology, 16(3), e1007651.

DNA copy-number measurement of genome replication dynamics by high-throughput sequencing: the sort-seq, sync-seq and MFA-seq family.

Batrakou, D., Müller, C., Wilson, R., Nieduszynski, C. (2020). Nature Protocols 15(3), 1255 - 1284.

Capturing the dynamics of genome replication on individual ultra-long nanopore sequence reads

Müller, C., Boemo, M., Spingardi, P., Kessler, B., Kriaucionis, S., Simpson, J., Nieduszynski, C. (2019).  Nature Methods 16(5), 429-436.

Deep functional analysis of synII, a 770-kilobase synthetic yeast chromosome.

Shen, Y., Wang, Y., Chen, T., Gao, F., Gong, J., Abramczyk, D., Walker, R., Zhao, H., Chen, S., Liu, W., Luo, Y., Müller, C., Paul-Dubois-Taine, A., Alver, B., Stracquadanio, G., Mitchell, L., Luo, Z., Fan, Y., Zhou, B., Wen, B., Tan, F., Wang, Y., Zi, J., Xie, Z., Li, B., Yang, K., Richardson, S., Jiang, H., French, C., Nieduszynski, C., Koszul, R., Marston, A., Yuan, Y., Wang, J., Bader, J., Dai, J., Boeke, J., Xu, X., Cai, Y., Yang, H. (2017).  Science (New York, NY) 355(6329), eaaf4791.