• Event
  • Scientific training

EI Long Read RNA Symposium 2021

This three-day symposium is aimed at researchers currently using long read technology for transcriptome annotation and analysis

Start date:

15 June 2021

End date:

17 June 2021

Time:

10h00 - 15h00

Venue:

Virtual (via Zoom)

Enquiries:

training@earlham.ac.uk

Registration deadline:

13 June 2021

Cost:

£15

About the event.

This three-day symposium will combine presentation of use cases of long read RNAseq, sample processing hints and tips from the experts in sequencing facilities at Earlham Institute and SciLifeLab National Genomics Infrastructure, and technology developments from leading long read technology companies.

There will also be opportunities to discuss topics such as annotation, differential expression, low input single cell applications, exchanging expertise and challenges with other participants.

Abstract submission:

We are offering the opportunity to present your research at the symposium, and abstracts should be submitted when you register. You will present either as a 10 minute talk or a poster, and can select your preference during the registration. The submission deadline for abstracts is Sunday 16 May 2021.

  • All abstracts must be submitted electronically as part of your registration. Submissions via email will not be accepted
  • Abstract limits are 250 words (excluding title, authors and affiliations)
  • Presenting author should be highlighted in bold
  • Abstracts will be reviewed within 14 days of the submission deadline and you will be informed of the outcome shortly thereafter

Who is this event for?

Research staff and students who are current or soon to be users of long read technology for the production of transcriptomic data and those analysing long read RNAseq data to produce genome annotations, differential expression analyses and those using RenSeq.

We particularly encourage early career researchers and PhD students to submit abstracts for selection for presentation.

This training forms part of our BBSRC National Capability in Advanced Training

Programme.

Please note that the below may be updated over the coming months and is subject to change. The times are in British Summer Time (BST). 

Day 1 - 15 June 2021

Time

Topic

09:45 - 10:00

Registrants Joining

10:00 - 10:15

Welcome and Introduction to Day 1
Wilfried Haerty, Earlham Institute

10:15 - 10:45

Sample Processing: The highs and the lows
Leah Catchpole, Earlham Institute

10:45 - 11:15

Single-cell Long Reads
Laura Mincarelli, Earlham Institute

11:15 - 12:00

Nanopore RNA sequencing: Live and Direct
Daniel Fordham, Oxford Nanopore Technologies

12:00 - 13:30

Lunch break

13:30 - 14:00

Sequencing DNA replication events using single molecules
Rosemary Wilson, Sir William Dunn School, University of Oxford

14:00 - 14:45

Applications of Long-Read HiFi Sequencing
Jonas Korlach, Pacific Biosciences

14:45 - 15:00

Close of Day 1

Day 2 - 16 June 2021

Time

Topic

09:45 - 10:00

Registrants Joining

10:00 - 10:15

Welcome and Introduction to Day 2
Wilfried Haerty, Earlham Institute

10:15 - 10:45

Long-read RNA sequencing from a core facility perspective
Adam Ameur, SciLifeLab National Genomics Infrastructure, Sweden

10:45 - 11:15

Annotation using very long reads (transcriptome)
David Swarbreck, Earlham Institute

11:15 - 11:45

Perturbed autoregulation of TDP-43 in neurodegeneration
Jemeen Sreedharan, King's College London

11:45 - 13:30

Lunch break

13:30 - 15:00

Discussion groups:
- Genome Annotation
- Differential Expression (gene and transcript) and Networks
- Sample processing and Low input single cell application

15:00 - 15:30

A guidelines for quality control, quantification and differential expression analysis using lrRNA-seq
Ana Conesa, Microbiology and Cell Sciences Department, University of Florida

Day 3 - 17 June 2021

Time

Topic

09:45 - 10:00

Registrants joining

10:00 - 10:15

Welcome and Introduction to Day 3:
Wilfried Haerty, Earlham Institute

10:15 - 10:45

Selected talks from abstracts:
Mariela Cortés-López, Institute of Molecular Biology, Mainz
Matt Tarnowski, University of Bristol

10:45 - 11:00

Break

11:00 - 11:30

“Taking the long (read) view of alternative splicing during cell differentiation”
David Wright, Earlham Institute

11:30 - 12:00

Detection of RNA modifications in single molecules
Tommaso Leonardi, Center for Genomic Science, Istituto Italiano di Tecnologia

12:00 - 13:30

Lunch break

13:30 - 14:00

Profiling of the canine brain transcriptome using high-throughput nanopore cDNA sequencing
Ola Wallerman, Uppsala University

14:00 - 14:30

Using long-read RNA sequencing to identify novel drug targets
Nicola Hall, University of Oxford

14:30 - 15:00

Closing remarks
Wilfried Haerty, Earlham Institute

Further information.

Please carefully review our standard online event booking terms and conditions prior to registering for this event. Completing an online registration and associated payment process will mean that you are bound by these terms and conditions. Any supplemental terms or changes to these conditions on a per event basis will be included on this page. If you have any queries regarding our events or in relation to your booking, please contact us at training@earlham.ac.uk