The newly established Technical Development group, led by Dr. Iain Macaulay, is developing new sequencing approaches to address a diverse range of biological problems.
One of the main focuses of the group is to establish and develop high-throughput single cell sequencing approaches. The group has considerable experience in single cell genomics, in particular in the development of novel “multi-omics” approaches - in which both the genome (or epigenome) and the transcriptome of a single cell can be assayed in parallel. Applying and extending such methods will provide key insights into biological heterogeneity at the single cell level.
The group has access to a number of platforms for single cell analysis, including a Fluidigm C1 and a “Facs-in-a-Petri”, as well as extensive laboratory automation solutions for implementation of multi-omics approaches such as G&T-seq.
The group also works closely with other faculty members as well as the platforms and pipelines team to ensure that new technologies and developments are implemented to support and enhance the scientific activity of the institute.
Decoding the regulatory network of early blood development from single-cell gene expression measurements
Moignard V, Woodhouse S, Haghverdi L, Lilly AJ, Tanaka Y, Wilkinson AC, Buettner F, Macaulay IC, Jawaid W, Diamanti E, Nishikawa S, Piterman N, Kouskoff V, Theis FJ, Fisher J, Göttgens B.
Nat Biotechnol. 2015 Mar;33(3):269-76. doi: 10.1038/nbt.3154. Epub 2015 Feb 9.
Chen L, Kostadima M, Martens JH, Canu G, Garcia SP, Turro E, Downes K, Macaulay IC, Bielczyk-Maczynska E, Coe S, Farrow S, Poudel P, Burden F, Jansen SB, Astle WJ, Attwood A, Bariana T, de Bono B, Breschi A, Chambers JC; BRIDGE Consortium, Choudry FA, Clarke L, Coupland P, van der Ent M, Erber WN, Jansen JH, Favier R, Fenech ME, Foad N, Freson K, van Geet C, Gomez K, Guigo R, Hampshire D, Kelly AM, Kerstens HH, Kooner JS, Laffan M, Lentaigne C, Labalette C, Martin T, Meacham S, Mumford A, Nürnberg S, Palumbo E, van der Reijden BA, Richardson D, Sammut SJ, Slodkowicz G, Tamuri AU, Vasquez L, Voss K, Watt S, Westbury S, Flicek P, Loos R, Goldman N, Bertone P, Read RJ, Richardson S, Cvejic A, Soranzo N, Ouwehand WH, Stunnenberg HG, Frontini M, Rendon A.
Science. 2014 Sep 26;345(6204):1251033. doi: 10.1126/science.1251033.
Macaulay IC, Voet T.
PLoS Genet. 2014 Jan 30;10(1):e1004126. doi: 10.1371/journal.pgen.1004126. eCollection 2014 Jan.
Sanjuan-Pla A, Macaulay IC, Jensen CT, Woll PS, Luis TC, Mead A, Moore S, Carella C, Matsuoka S, Bouriez Jones T, Chowdhury O, Stenson L, Lutteropp M, Green JC, Facchini R, Boukarabila H, Grover A, Gambardella A, Thongjuea S, Carrelha J, Tarrant P, Atkinson D, Clark SA, Nerlov C, Jacobsen SE.
Nature. 2013 Oct 10;502(7470):232-6. doi: 10.1038/nature12495. Epub 2013 Aug 11.
Characterization of transcriptional networks in blood stem and progenitor cells using high-throughput single-cell gene expression analysis
Moignard V, Macaulay IC, Swiers G, Buettner F, Schütte J, Calero-Nieto FJ, Kinston S, Joshi A, Hannah R, Theis FJ, Jacobsen SE, de Bruijn MF, Göttgens B.
Nat Cell Biol. 2013 Apr;15(4):363-72. doi: 10.1038/ncb2709. Epub 2013 Mar 24.